Clinical Application of Miniature Peroneal Perforation Flap for Free Grafting In Soft Fibers of Hand, Foot and Skin

Objective: To study the clinical application and effect of free transplantation of miniature peroneal perforation flap in soft and tissue defects of hand, foot and skin. Method: 28 patients with hand, foot, skin and soft tissue defects who were treated in hospital from May 2015 to May 2016 were selected as the subjects. The patients were randomly divided into group A as experimental group and group B as control group. Two groups of patients with treatment site microcirculation, postoperative recovery and sensory score were compared. Results: The microcirculation indexes of group A were significantly better than those of group B (P <0.05). The rate of recovery in group A was 92.86% and rate of recovery in group B was 71.43%, after treatment recovery in group A is significantly better than group B (P <0.05). Before and after treatment, the sensory scores of the two groups were not statistically significant (P> 0.05). The sensory score of group A was significantly higher than that of group B (P <0.05). Conclusion: The microperitoneal perforation flap dissection has a good clinical effect, which can effectively improve the postoperative recovery of patients with soft tissue defects of hand, foot and skin, improve the microcirculation index of the treatment site and improve the sensory score of the treatment site, should be widely used in clinical treatment

Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding

Background In late December, 2019, patients presenting with viral pneumonia due to an unidentified microbial agent were reported in Wuhan, China. A novel coronavirus was subsequently identified as the causative pathogen, provisionally named 2019 novel coronavirus (2019-nCoV). As of Jan 26, 2020, more than 2000 cases of 2019-nCoV infection have been confirmed, most of which involved people living in or visiting Wuhan, and human-to-human transmission has been confirmed. Methods We did next-generation sequencing of samples from bronchoalveolar lavage fluid and cultured isolates from nine inpatients, eight of whom had visited the Huanan seafood market in Wuhan. Complete and partial 2019-nCoV genome sequences were obtained from these individuals. Viral contigs were connected using Sanger sequencing to obtain the full-length genomes, with the terminal regions determined by rapid amplification of cDNA ends. Phylogenetic analysis of these 2019-nCoV genomes and those of other coronaviruses was used to determine the evolutionary history of the virus and help infer its likely origin. Homology modelling was done to explore the likely receptor-binding properties of the virus. Findings The ten genome sequences of 2019-nCoV obtained from the nine patients were extremely similar, exhibiting more than 99·98% sequence identity. Notably, 2019-nCoV was closely related (with 88% identity) to two bat-derived severe acute respiratory syndrome (SARS)-like coronaviruses, bat-SL-CoVZC45 and bat-SL-CoVZXC21, collected in 2018 in Zhoushan, eastern China, but were more distant from SARS-CoV (about 79%) and MERS-CoV (about 50%). Phylogenetic analysis revealed that 2019-nCoV fell within the subgenus Sarbecovirus of the genus Betacoronavirus, with a relatively long branch length to its closest relatives bat-SL-CoVZC45 and bat-SL-CoVZXC21, and was genetically distinct from SARS-CoV. Notably, homology modelling revealed that 2019-nCoV had a similar receptor-binding domain structure to that of SARS-CoV, despite amino acid variation at some key re